A stock breeder ( or a cultivator ) who , by doing crossing , is bet for newfangled varieties , really only wants one thing : spry and well-to-do brainwave into the amount of DNA and chromosome that a piece of plant material contains .
After the takeover of Partec by Sysmex in 2013 , Partec ’s catamenia cytometer , with client convenience in mind , has been further grow so that now a full software system with ironware , specific software and reagents can be offered .
Cyflow ploidy analyser and Space
It also includes the late set of reagent for ploidy and chromosome size of it determination : CyStain UV OxProtect and CyStain PI OxProtect . " With the new reagents that are brought like a shot to the industrial plant material to be able to quantify , air can no longer attain a piece of leaf and therefore no oxidization can occur , " says Kars de Wildt of Sysmex - Partec , responsible for sale of the technology in the Benelux . “ Every breeder has a number of multifariousness where oxidisation is a problem . No oxidisation means less oxidation randomness and a decipherable readable result for the stock breeder and/or grower , make a ploidy determination or chromosome measure more interpretable . ”
Photo right : CyStain PI OxProtect
With UVBut first back to " early times " , because flow rate cytometers have been around for decade ( Partec launch the first flow cytometer on the market in 1969 ) , just like Partec and Sysmex themselves . Where Partec , which originated in 1960 , is well known thanks to the fact that almost all major stock breeder do have " a Partec " in - house , Sysmex is middling less so as provider of in - vitro symptomatic product . But many specialists in the market place will know that Sysmex works with * * * * ultraviolet lamps in their system where many other parties in the grocery work with ( much more expensive ) laser .
“ Our reagent are UV - adjusted and that is where we tell apart ourselves , ” say Kars before explain something about the history of Sysmex . “ Sysmex was base in 1968 and its core clientele is hematology , a medical specialty that deals with abnormalities of the bloodline , using fluorescence flow - based prison cell applied science . Sysmex Benelux also has a declamatory offshoot with an Industry department , which includes Sysmex Partec , which now also offer total solution in horticulture . "
Sysmex Partec Space
Do it yourselfWhere menses cytometry has long been regarded as complicated and complex , and that still may be the instance when you go into item , over the years the engineering has been simplified by Sysmex Partec .
“ breeder and grower who now outsource ploidy and/or genome size determination should take a look at the number of tests they have had carried out and whether it is worthwhile to buy a system themselves , ” says Kars . “ Nowadays you see that it is sure of interest for medium - sized companies , now that it is also gentle to do it yourself . Regardless of the knowledge level of the client and the country where the stock breeder / agriculturist is locate , the technology , the total solution is wanton to use . ”
accord to Kars , a company does not call for to have a complicated laboratory . “ I know customer , also here in the Netherlands , who pre - treat , filter and measure sample distribution at the kitchen table all day long . ”
Ploidy determination with a tomato plant sampling
Ensure qualityKars sees many development in the area of breed at customers , for example in the breeding of melon . “ When you look at that this was once a star fruit , you have it off that a lot has happened in the discipline of DNA . But you’re able to also see the same in cucumber , bell pepper , gerbera and in fact any harvest , because a different colour or a new resistance makes everything slightly different in terms of DNA . ”
Moreover , the technology is not only authoritative for research intention and the development of novel varieties , but evenly good for issue timber labels , Kars say . " With a miscellany from which the grower receives ejaculate , the cultivator , like the stock breeder , course wants to be sure whether the seed meet all requirement and whether it is a diploid ( double chromosome pair ) or a tetraploid ( four chromosome pairs ) . "
Finally , with the tardy developments as described above , Kars would wish to say that companionship that still have a Partec flow cytometer from before 2013 can have their organization checker by a trained engineer from the manufacturer Sysmex Partec . “ Companies can adjoin us for that . Also for the verbatim provision of reagents , we are also extra attached to the total solution with avail and advice from A to Z. "
For more information : Sysmex Partecwww.sysmex.nl
Kars de Wildt[email protected]+31 ( 0 ) 623781465
© FloralDaily.com/Thijmen Tiersma
